Summerlee Science Complex Rm 1401,
University of Guelph
488 Gordon Street
Guelph, Ontario, Canada N1G 2W1
Phone: 519-824-4120 ext. 58357
Fax: 519-767-1656
Email: genomics@uoguelph.ca
Website: https://www.uoguelph.ca/aac/
facilities/genomics
Dy
e Term
inator Cy
cle S
e
que
n
ci
ng Re
qui
sition Form
Client Name Position
Email Department
Phone Supervisor
Authorized Signature (required): ________________________________________________
Billing Information GL Coding (26 digits)
Fund (3) Unit (6) Grant (6) Project (6) Object (5)
64251
Special Requests print electropherogram
keep sample for return
Primer Description
Dilute primers to 5uM and provide 2uL for each reaction ΔT
m
(
C) = [4
(G+C)+2
(A+T)]-3
Primer Name Primer Sequence
Annealing
Temperature
Concentration
(pmol/ul)
1 5’
2 5’
3 5’
4 5’
5 5’
6 5’
Template Description
Sample Name Primer number
Vector
size (bp)
Insert or PCR
product size (bp)
Concentration
(ng/ul)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
DNA Tem
plate
Size
Concentration
(ng/uL)
Quantity
(ng/rxn)
100-500bp
5-10 20
500-1000bp
10-30 50
1kb-5kb
30-100 1
50
5kb-10kb
100-200
300
Template Preparation
Plasmid DNA must be free of protein and bacterial DNA
PCR products must be purified using various methodologies
UV exposure of excised bands must be minimal
Recommend quality/quantity assessment with Nanodrop
AAC Dye Terminator Order Form - Internal (
08/31/18)
click to sign
signature
click to edit
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