Science Complex Rm 1401,
University of Guelph
488 Gordon Street
Guelph, Ontario, Canada N1G 2W1
Phone: 519-824-4120 ext. 58357
Fax: 519-767-1656
Email: genomics@uoguelph.ca
Website: http://www.uoguelph.ca/~genomics/
AAC Dye Terminator Order Form - Internal (11/18/10)
Dye Terminator Cycle Sequencing Requisition Form
Client Name Position
Email Department
Phone Supervisor
Authorized Signature (required): ________________________________________________
Billing Information GL Coding (26 digits)
Fund (3) Unit (6) Grant (6) Project (6) Object (5)
64251
Special Requests □ print electropherogram □ keep sample for return
Primer Description
Dilute primers to 5uM and provide 2uL for each reaction ΔT
m
(
◦
C) = [4
◦
(G+C)+2
◦
(A+T)]-3
◦
Primer Name Primer Sequence
nnealing
Temperature
Concentration
(pmol/ul)
1 5’
2 5’
3 5’
4 5’
5 5’
6 5’
Template Description
Sample Name Primer number
ector
size (bp)
Insert or PCR
product size (bp)
Concentration
(ng/ul)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
DNA Template
Size
Concentration
(ng/uL)
Quantity
(ng/rxn)
100-500bp
5-10 20
500-1000bp
10-30 50
1kb-5kb
30-100 150
5kb-10kb
100-200 300
Template Preparation
9 Plasmid DNA must be free of protein and bacterial DNA
9 PCR products must be purified using various methodologies
9 UV exposure of excised bands must be minimal
9 Recommend quality/quantity assessment with Nanodrop
