Science Complex Rm 1401,
University of Guelph
488 Gordon Street
Guelph, Ontario, Canada N1G 2W1
Phone: 519-824-4120 ext. 58357
Fax: 519-767-1656
Email: genomics@uoguelph.ca
Website: http://www.uoguelph.ca/~genomics/
AAC Dye Terminator Order Form - Internal (11/18/10)
Dye Terminator Cycle Sequencing Requisition Form
Client Name Position
Email Department
Phone Supervisor
Authorized Signature (required): ________________________________________________
Billing Information GL Coding (26 digits)
Fund (3) Unit (6) Grant (6) Project (6) Object (5)
64251
Special Requests print electropherogram keep sample for return
Primer Description
Dilute primers to 5uM and provide 2uL for each reaction ΔT
m
(
C) = [4
(G+C)+2
(A+T)]-3
Primer Name Primer Sequence
A
nnealing
Temperature
Concentration
(pmol/ul)
1 5’
2 5’
3 5’
4 5’
5 5’
6 5’
Template Description
Sample Name Primer number
V
ector
size (bp)
Insert or PCR
product size (bp)
Concentration
(ng/ul)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
DNA Template
Size
Concentration
(ng/uL)
Quantity
(ng/rxn)
100-500bp
5-10 20
500-1000bp
10-30 50
1kb-5kb
30-100 150
5kb-10kb
100-200 300
Template Preparation
9 Plasmid DNA must be free of protein and bacterial DNA
9 PCR products must be purified using various methodologies
9 UV exposure of excised bands must be minimal
9 Recommend quality/quantity assessment with Nanodrop
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