Summerlee Science Complex Rm 1401
University of Guelph
488 Gordon Street
Guelph, Ontario, Canada N1G 2W1
Phone: 519-824-4120 ext. 58357
Fax: 519-767-1656
Email: genomics@uoguelph.ca
Website: https://www.uoguelph.ca/aac/
facilities/genomics
Dye Terminator Cycle Sequencing
Requisition Form
Purchase Order Number
Client Name Invoice Address:
Address
Phone
Email
Results Printed electropherogram Email .txt / ab1 files
Provided Disc
Primer Description
Dilute primers to 5pmol/ul and provide 10pmol/reaction ΔTm(C) = [4(G+C)+2(A+T)]-3
Name Primer Sequence Annealing
Temperature
Concentration
(pmol/ul)
1 5’
2 5’
3 5’
4 5’
5 5’
6 5’
Template Description
Sample Name Sequence
with primer
number
ds/ss
DNA/RNA
Vector
size (bp)
Insert or PCR
product size
(bp)
Concentration
(ng/ul)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
DNA Concentration
(ng/ul)
Quantity
(ng)
dsDNA 1-10kb bp
5-200 300
PCR 100-500bp 1-10 20
PCR 500-1000bp
10-30 50
PCR 1000-5000bp 25-50 150
Recommended DNA Concentrations and Quantities
Plasmid DNA must be free of protein and bacterial DNA
PCR products must be purified using various methodologies
UV exposure of excised bands must be minimal
AAC Dye Terminator Order Form - External (08/31/18)
Chrome Web Store
It looks like you haven't installed the Fill Chrome Extension Add to Chrome