Instructions for Submission of Specimens for 2019‐nCoV (COVID-19)
Testing at the New York City Public Health Laboratory (PHL)
NOTE: These instructions apply AFTER approval has been given (via conference call with a medical epidemiologist from the
New York City Department of Health and Mental Hygiene {NYC DOHMH}) to test a patient for 2019‐nCoV (COVID-19) virus
infection. Please call the Provider Access Line at 1‐866‐692‐3641 for consultation or to report a suspected 2019-nCoV
(COVID-19) case. When 2019‐nCoV testing has been authorized, hospitals may request a specimen collection kit from the PHL.
1. Complete the requisition form (“Laboratory Test Request” form)
• No specimen will be tested without a completed “Laboratory Test Request” form.
• EACH SPECIMEN requires its own form.
For example, if you are submitting two upper respiratory tract specimens, oropharyngeal and
nasopharyngeal swabs combined in one Viral Transport Medium (VTM) tube and a lower respiratory
specimen for the same patient, you need to complete TWO forms, one for each specimen.
• All starred (*) fields are REQUIRED.
Failure to complete all required fields will result in delays in testing or the specimen may be rejected.
• Place the form inside the outer pouch of the specimen bag.
• Place only one 2019-nCoV (COVID-19) specimen and one form per bag.
2. Collect both specimen types
• Upper respiratory tract swabs: collect ONE Nasopharyngeal (NP) swab and ONE Oropharyngeal (OP)
swab, and combine them into ONE VTM tube. Swabs must be synthetic (non‐cotton), non-absorbent,
and flocked, and placed in liquid VTM. Swabs NOT in VTM are NOT acceptable. Place the NP swab and
the OP swab together in ONE tube and submit one form for this combined OP and NP specimen.
• Lower respiratory tract specimen (sputum, tracheal aspirate or bronchoalveolar lavage).
For detailed instructions on specimen collection, please refer to “Interim Guidelines for Collecting,
Handling, and Testing Clinical Specimens from Patients Under Investigation (PUIs) for Coronavirus Disease
2019 (COVID-19)": https://www.cdc.gov/coronavirus/2019-nCoV/guidelines-clinical-specimens.html
3. Label the specimen
• Failure to properly label the specimen will result in rejection and the specimen will not be tested.*
• Specimen tubes MUST be labeled with:
o Patient’s First and Last Names
o Patient’s Date of Birth
o Date of C
ollection
o Specimen Source
• All information
on the specimen label must EXACTLY MATCH the information on the Laboratory Test
Request form, including the spelling of the patient’s first and last names.
4. Specimen handling
• Keep specimens refrigerated while awaiting transport.
• Transport with cold ice packs if the specimens are transported within 72 hours from the time of
specimen collection. If exceeding 72 hours, freeze at ‐70°C and transport on dry ice.
• Follow shipping regulations for UN 3373 Biological Substance, Category B when sending potential
2019‐nCoV (COVID-19) specimens.