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BIOSAFETY LABORATORY INSPECTION/AUDIT CHECKLIST (Marine Biological Laboratory)

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File type: PDF

U.S.A. forms for Marine Biological Laboratory

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Page 1 of 3
Biosafety Lab Inspection/Audit Checklist- February 2018
BIOSAFETY LABORATORY INSPECTION/AUDIT CHECKLIST
Inspected By (Name):
Date:
Principal Investigator or Course Director:
Center or Course:
Building:
Room(s):
Laboratory Supervisor:
Phone:
ITEM
YES
NO
COMMENTS
IBC APPROVALS AND RESOURCES
Research Safety Questionnaire completed.
Biological research protocol approved by IBC (provide
approval date).
Any amendments to IBC approved research protocol is
updated and reviewed by IBC.
Lab-specific biosafety manual and SOPs available.
Lab safety training current for all laboratory personnel.
STANDARD MICROBIOLOGICAL PRACTICES
Access to laboratory is restricted
Laboratory door has proper biohazard signage (e.g., contact
information and emergency numbers) provided by EHS.
Hands are washed after working with biohazards and before
leaving the laboratory.
Eating, drinking, storing food/drinks, applying cosmetics, and
tobacco use are prohibited in laboratory areas.
Mouth pipetting is prohibited.
Needles are never reused, recapped, bent or broken before
disposal.
Plastic-ware is substituted for glassware whenever possible.
Procedures involving aerosol or splash generation are
minimized
Work surfaces are decontaminated after completion of work.
Samples are put in a durable, leak-proof container for
storage or transport.
Biohazard stickers are affixed on all equipment involving
biohazards.
Biological spill kit is available.
Page 2 of 3
Biosafety Lab Inspection/Audit Checklist- February 2018
ITEM
YES
NO
COMMENTS
BIOLOGICAL WASTE PROCEDURES AND PRACTICES
Bench paper properly disposed of after each use.
Biological waste (e.g., cultures, stocks, media, tissues,
plates) is properly decontaminated before disposal
Biological waste is kept in a secondary container for storage
and transport.
Biological waste and sharps containers are not overfilled.
No biohazards in regular trash or in non-hazardous glass
waste containers.
Needles, syringes, and other sharps are disposed of in a
plastic biohazard sharps container.
Non-contaminated broken glassware is disposed of in
cardboard glass waste container.
PRIMARY CONTAINMENT BARRIERS AND PPE
Proper PPE is worn while working with biohazards (minimum
requirement is gloves and laboratory coat; additional PPE
may be required depending on agent or procedure).
Mucous membrane protection is worn when aerosol
generation is possible.
No open-toe shoes worn in laboratory.
Biological Safety Cabinet is currently certified (annual
certification).
Biological Safety Cabinet is clean and free of clutter.
PPE is not worn while handling personal devices (cell
phone, computers, iPods, etc.)
Chemical fume hood is currently certified (annual
certification sticker).
Chemical fume hood is not used to process biological
materials.
Centrifuges, vortex mixers, incubators, shakers, etc. are
clean and in good working condition.
LABORATORY FACILITIES
Laboratory has sink and soap and for hand washing.
Eyewash station readily available (10 seconds, walking).
Emergency shower readily available.
Benchtops are impervious to water and easily cleaned.
No cloth furniture or carpets present.
Gas cylinders are secured with chain to wall.
If windows can open, they are fitted with screens.
Page 3 of 3
Biosafety Lab Inspection/Audit Checklist- February 2018
ITEM
YES
NO
N/A
COMMENTS
BIOSAFETY LEVEL 2 (BSL-2) SPECIAL PRACTICES
All personnel completed BSL-2 training.
Procedures involving aerosol or splash generation are minimized or
performed in a biosafety cabinet.
Lab equipment is routinely decontaminated.
No animals or plants unrelated to research are present.
Biosafety cabinet located away from heavily traveled areas
(doorways, etc.).
Vacuum lines properly setup with clean HEPA filters and appropriate
disinfectant.
Centrifuges are used with safety cups to reduce aerosol hazard.
Personnel are familiar with post-exposure evaluation and follow-up.
OSHA BLOODBORNE PATHOGENS (i.e., Human Cell Lines,
Bodily Fluids, Tissues)
MBL Bloodborne Pathogen Exposure Control Plan available.
Personnel have completed annual BBP training.
Personnel have been offered Hepatitis B vaccination or signed
declination form.
SHIPPING BIOLOGICAL MATERIALS
Personnel have completed DOT/IATA biological shipping training
within the past 2 years.
CORRECTIVE ACTIONS:
RECOMMENDATIONS:
Principal Investigator or Course Director: ________________________ Date: _________
Biosafety Officer: ____________________________________________ Date: _________
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BIOSAFETY LABORATORY INSPECTIONAUDIT CHECKLIST (Marine Biological Laboratory)

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Completed 16 August 2020

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