SECTION 3: ANIMAL PROCEDURES
3.1 Please provide a list of specific procedures that involve the use of animals in this project.
Where appropriate, this section should clearly identify specific procedures intended to
minimize pain such as anesthetic, analgesic or tranquilizing drug application. Note: an
annual report of the actual number of animals collected by species must be provided to the IACUC.
3.2 Check which of the following pain categories applies to the procedures to be used on
animals in this project. Note: letter designations correspond to the eventual USDA report; where A and
F are reserved to report the numbers, types, and totals of affected animals.
____ B – animals collected and bred/held as part of the project
____ C – procedures will cause no (or minimal) pain or distress (e.g. identification
and measurements, injections, blood sampling, tattooing, electrofishing, live-trapping,
netting, marking by fin-clip or toe-clip, health assessments,…)
____ D – procedures involve pain or distress but anesthetics or tranquilizing
drugs are used
____ E* – procedures involve pain or distress and no anesthetic, analgesic or
tranquilizing drugs can be used (e.g. leg-hold traps, confinement in adverse
environments, or procedures with death as an endpoint, like rotenone sampling for fish)
* If any category “E” procedures will be performed, you must complete sub-section 3.3 below and 3.4 if
applicable.
3.3 Justification for special circumstances (USDA pain category “E” procedures only).
Projects involving USDA pain category “E” procedures require specific scientific
justification of why the procedure is necessary without mitigation.