OFFICE FOR RESEARCH USE ONLY
DATE RECEIVED OR REVISED:
VERSION:
*ONLY TYPED FORMS WILL BE ACCEPTED*
If this protocol is a resubmission, which protocol(s) is it intended to replace: Old IBC Protocol No.(s):
DATE APPLICATION COMPLETED: IBC No. (Will be assigned initially by IBC office):
1. PROJECT TITLE:
2. PRINCIPAL INVESTIGATOR:
Name (Last, First):
Campus OR Work Phone Number:
Department Affiliation: Mailing Address
1
: Choose One, if Applicable:
New Address - Change for all other
active protocols
E-mail Address (use ISU email
if ISU employee or student):
1
For on campus investigators use Department and Mail Code. For off campus investigators provide complete mailing address.
3. PERSONNEL for CORRESPONDENCE: List personnel below who should be copied on the correspondence.
Name (Last, First): Name (Last, First): Name (Last, First):
Campus or Work Phone Number: Campus or Work Phone Number: Campus or Work Phone Number:
Department Affiliation: Department Affiliation: Department Affiliation:
E-mail Address (use ISU email if ISU employee
or student)
:
E-mail Address (use ISU email if ISU
employee or student)
:
E-mail Address (use ISU email if ISU
employee or student)
:
Mailing Address
1
: Mailing Address
1
: Mailing Address
:
Choose One, if Applicable:
New Address - Change for all protocols
Choose One, if Applicable:
New Address - Change for all protocols
Choose One, if Applicable:
New Address - Change for all protocols
Research Coordinator (Not Directly
Involved with rDNA/IA)
Research Coordinator (Not Directly
Involved with rDNA/IA)
ALL personnel who work directly with rDNA, synthetic DNA, or infectious agents
must be listed in Personnel Section (VII).
FORM A - Protocol for Use of
Recombinant or Synthetic Nucleic
Acid Molecules in Research
Version 1.0
Idaho State University, Office for Research
Institutional Biosafety Committee (IBC)
1651 Alvin Ricken Drive, Pocatello, ID 83201-8046
Phone: 208-282-2179 Fax: 208-282-4723
Page 1 of 8 Document #
ISU Protocol for Use of Recombinant DNA in Research - Form A/IBC
IBC Protocol No. ________
Version 1.0
I. Review Category
Based on the review categories defined in appendix X (change to Form A Instructions?) of this form, this protocol
falls under:
Review Category 1 Review Category II Review Category III Review Category IV
II. Background
a. Purpose of the research project:
Describe the overall purpose of the project in a few sentences. Use language and/or define scientific terminology in
lay terms:
b. Scientific background:
Describe the scientific background and expertise of the personnel listed in this protocol:
Page 2 of 8 Document #
III. Project Description - Table 1 - Plasmids, Vectors, and Genes
Plasmids/Source
Antibiotic
Resistance
Gene
Gene/Host/Source Name/Function
Host
Risk
Group
III. Project Description - Table 2 - Bacterial Strains
Strain/Source Relevant Characteristics, Attenuation, etc
Native Antibiotic
Resistance Gene
Risk
Group
Plasmids To Be
Transfected
Page 3 of 8 Document #
ISU Protocol for Use of Recombinant DNA in Research - Form A/IBC
IBC Protocol No. ________
Version 1.0
III. Project Description - Table 3 Non-Bacterial Strains and Cell Lines
Strain/Source Relevant Characteristics
Native Antibiotic
Resistance Gene
Risk
Group
Plasmids To Be
Transfected
III. Project Description - Table 4 - Viral Packaging and Attenuation
Vector Class and
Name
Source
Packaging
Cell Line
Host Range Post Packaging
Replication
Defective
Relevant Characteristics/Attenuation Mechanism
Page 4 of 8 Document #
III. Project Description -
Provide a description of the in vitro work conducted with the reagents listed in tables 1-4 above
Page5 of 8 Document #
ISU Protocol for Use of Recombinant DNA in Research - Form A/IBC
IBC Protocol No. ________
Version 1.0
IV. Animal Work
a. Transgenic/Knockin/Knockout Generation of Rodent and Nonrodent species- Provide a complete
description of the construct and species that will be used for production of transgenic/knockin/knockout
animals. Indicate whether PI or another source will produce these animals.
b. If transgenic/knockin/knockout animals will be purchased, transferred, or existing lines will be crossed-
Provide a description below.
c. All other use of rDNA/synthetic DNA in Animals-
V. Plant Work
a. Transgenic Production- Provide a description of the construct that will be used for production of transgenic
plants.
b. All other use of rDNA/synthetic DNA in Plants-
Page 6 of 8 Document #
ISU Protocol for Use of Recombinant DNA
in R
esearch - F
o
rm A/I
BC
IBC Protocol No. ________ Version 1.0
VI. Additional Information
a. Indicate the location of ALL lab(s)/rooms in which the work will take place:
Rm No. Bldg Rm No. Bldg Rm No. Bldg
b. If applicable, indicate in which animal facility animal work will take place and type of room in which animals will be
maintained. Facility Name.
BSL1 Standard Housing
c. Indicate the location of ALL lab(s) in which the rDNA molecules/infectious agents will be stored:
Check here if same as VIa
Rm No.
Rm No. Bldg
d. Indicate the highest biosafety level (BSL) required for this project: 1 2
e. Will shipping off-campus of any potentially infectious biological material (PIBM) be done? Yes No
Name of person(s) responsible for shipping:
VIIa. Personnel - List the following information for all personnel involved with this protocol.
#1 1) Full Name: 2) Email: 3) Degree: 4) BST Date: 5) BBP Date: 6) ST Date:
7) Procedures/Experience with Procedures: 8) Trainer:
#2 1) Full Name: 2) Email: 3) Degree: 4) BST Date: 5) BBP Date: 6) ST Date:
7) Procedures/Experience with Procedures: 8) Trainer:
#3 1) Full Name: 2) Email: 3) Degree: 4) BST Date: 5) BBP Date: 6) ST Date:
7) Procedures/Experience with Procedures: 8) Trainer:
#4 1) Full Name: 2) Email: 3) Degree: 4) BST Date: 5) BBP Date: 6) ST Date:
7) Procedures/Experience with Procedures: 8) Trainer:
#5 1) Full Name: 2) Email: 3) Degree: 4) BST Date: 5) BBP Date: 6) ST Date:
7) Procedures/Experience with Procedures: 8) Trainer:
Page 7 of 8
Document #
Bldg
ISU Protocol for Use of Recombinant DNA in Research - Form A/IBC
IBC Protocol No. ________
Version 1.0
VIIb. Personnel Training
Describe the training provided to your personnel. For protocols involving biosafety level 2, please describe all
additional training.
VIII. Assurances
The Principal Investigator assures that the use of all rDNA, potentially infectious agents, and infectious agents will
be conducted in accordance with the ISU Institutional Biosafety Committee Policy.
Signature of Principal Investigator
Date
Typed or printed PI name:
Page 8 of 8 Document #
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signature
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