Fillable BiosafetyManualBSL1Sept16-15v2 ISU IBC BSL1 Manual

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BiosafetyManualBSL1Sept16-15v2 ISU IBC BSL1 Manual

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Principal Investigator:
Laboratory Location:
DO NOT USE THIS TEMPLATE IF ANY OF THE FOLLOWING IS TRUE:
1. Human or Nonhuman primate cells, cell lines, or tissues are used
2. Viral vectors are used
3. Infectious agents are used
The BSL 2 Laboratory Biosafety manual must be completed for work involving these
agents. For BSL3 agents, contact IBC.
Standard Microbiological Practices
1. A Universal biohazard symbol will be posted at the entrance to the laboratory when infectious
agents or materials that are handled at BSL1 are present. The biohazard label will read
“Biohazard” “Low Risk.”
2. A Laboratory Door Sign will be posted at the laboratory entrance. The sign will include the
following information:
a. Room, Building, Department, and Date.
b. Room Use/Description such as Research Laboratory.
c. The types of potential hazards present in the laboratory will be checked off on the sign.
For Biological Hazards the terminology “Low Risk” will be checked off. The specific type
of agent in use will not be listed.
d. Emergency Contact information for the following personnel will be posted on the door sign:
3. Entry to the laboratory is subject to approval of the Principal Investigator listed above when
work
with potentially biohazardous material is being conducted.
4. All personnel working with rDNA or infectious agents at BSL1 must wash their hands after
working with potentially hazardous materials and before leaving the laboratory. Keep hands
away from the mouth, nose, eyes, face and hair.
5. Good Laboratory Practices will be followed at all times.
a. Do not eat, drink, chew gum, smoke, handle contact lenses, or apply cosmetics in any
laboratory area.
b. All food for human consumption must be stored outside the laboratory area in cabinets or
refrigerators designated for this purpose.
c. Mouth pipetting is prohibited. Mechanical pipetting devices must be used.
d. Do not store personal items such as coats, boots, bags and books in the laboratory.
6. Personnel in the laboratory will use the following personnel protective equipment when
working with potentially infectious materials:
BSL 1 Laboratory
Biosafety Manual
Version 1.0
Idaho State University, Office for Research
Institutional Biosafety Committee (IBC)
1651 Alvin Ricken Drive, Pocatello, ID 83201-8046
Phone: 208-282-2179 Fax: 208-282-4723
Page 1 of 5
ISU BSL1 Laboratory Biosafety Manual/IBC
PI:
Version 1.0
a. At a minimum disposable gloves and lab coats, gowns, or uniform must be used.
b. Personnel wearing contacts will be advised to wear eye protection when working with
potentially infectious material.
c. Personnel will change gloves when they are contaminated, compromised, at conclusion of
work, or more frequently if required. Gloves will be disposed
d. Gloves will not be reused.
e. List any additional PPE that will be used: or Check N/A
7. Sharps, such as needles, scalpels, pipettes, and broken glassware will be handled in the
following manner:
a. Whenever possible, use of sharps with potentially hazardous material will be avoided.
Plasticware will be substituted for glassware whenever possible.
b. The handling of sharps will be minimized. Needles will not be bent, sheared, broken,
recapped, removed from disposable syringes, or otherwise manipulated by hand before
disposal.
c. Used disposable needles and syringes will be carefully placed in a puncture-resistant
containers used for sharps disposal. Sharps containers will not be beyond ¾ full.
d. Non-disposable sharps will be placed in a hard walled container for transport to a
processing area for decontamination. Method of decontamination:
i. A decontamination solution may be used in the container, but instruments must be sterilized
(autoclaved) before reuse.
e. Biohazardous sharps will be disposed when containers are 2/3 to 3/4 full. The TSO will be
contacted to arrange disposal of biohazard sharps.
f. Broken glassware will not be handled directly. It will be removed using a brush and dustpan,
tongs, or forceps and properly disposed of glassware waste.
g.
Additional precautions: or Check N/A
8. All procedures will be conducted such that the creation of splashes and/or aerosols is
minimized. Work may be conducted on the open bench; however, if procedure produces
excessive aerosols (e.g. sonication), use of a biosafety cabinet will be considered and eye
protection.
9. Work surfaces will be decontaminated after completion of work and after any spill or splash of
potentially infectious material.
10% final concentration of household bleach made daily followed by 70% ethanol to remove
bleach residue.
Chlorine Dioxide. Final concentration:
Other – List agent and concentration:
10. Spills will be handled in accordance with ISU Hazardous Waste Policy Manual
(http://www.physics.isu.edu/health-physics/tso/hazman2008complete.pdf). All personnel will
be familiar with these procedures.
Page 2 of 5
ISU BSL1 Laboratory Biosafety Manual/IBC
PI:
Version 1.0
11. Liquid biohazardous waste must be decontaminated with appropriate disinfectant prior to
disposal.
10% final concentration of household bleach
Other – List agent and final concentration:
Not Applicable.
Contact time must be at least 30 minutes.
Following decontamination, liquid may be disposed of down the sink and the sink rinsed with
water.
12. Solid waste that has been in contact with potentially infectious materials will be disposed of in
the following manner:
Low Risk Waste (BSL1 Waste – for this protocol)
NOTE: ALL waste generated by the laboratory within a specific location must qualify as low
risk. IF not ALL waste generated in that location much be treated as moderate risk.
Autoclaved and disposed – All biohazardous waste is placed in biohazard bag, biohazard
bag is placed in autoclavable container (i.e., polypropylene tray), and transported to autoclave
on a cart. Waste is autoclaved in the tray and then placed in black plastic bag and discarded
in standard waste.
Commercial. All biohazardous waste is placed in biohazard bag inside solid biohazard
container. TSO approved commercial biohazard disposal company retrieves and disposes
biohazard waste in compliance with State and Federal regulation.
13. When potentially infectious materials need to be transported outside the laboratory for
decontamination (such as transport to the autoclave), the materials will be placed in a
durable, leak proof container and secured for transport.
14. If potential infectious materials will be shipped from the facility, the technical safety office must
be contacted for guidance, x2310. All appropriate local, state and federal (U.S. Department
of Transportation) regulations must be followed. For air or international shipments,
International Air Transportation Association (IATA) rules must be followed. Training is
required for packaging, labeling and shipping of all infectious materials. Contact the
Technical Safety Office for training on shipping and receiving dangerous goods.
15. All potential exposure incidents of exposure or accidents involving potentially infectious
material will be reported to the Principal Investigator/Laboratory Supervisor and the
PI/Laboratory Supervisor will submit an incident report to the IBC.
16. All laboratory personnel will be informed of these biosafety practices and procedures and the
principal investigator/laboratory supervisor will ensure that laboratory personnel receive
appropriate training regarding their duties, the necessary precautions to prevent exposures,
and exposure evaluation procedures.
17. Documentation of training will be maintained in the laboratory and personnel will receive
annual updates or additional training when procedural or policy changes occur.
Page 3 of 5
ISU BSL1 Laboratory Biosafety Manual/IBC
PI:
Version 1.0
18. All personal are to be instructed that their health status may have an impact on their
susceptibility to infection and, if required, their ability to receive immunizations or prophylactic
interventions. Therefore, all laboratory personnel and particularly women of childbearing age
will be provided with information regarding immune competence and conditions that may
predispose them to infection. Personnel that have conditions that would render them more
susceptible to infection or who are pregnant, will be encouraged to self-identify to these
issues to the Principal Investigator and their personal physician, such that appropriate
counseling and guidance can be provided.
The Safe Use of Autoclaves
1. There are many different types of autoclaves used on the ISU campus. It is the responsibility
of the supervisor to ensure that all authorized individuals are properly trained on the use of
the autoclave(s) used by laboratory personnel.
2. The autoclaves used by this laboratory are listed in the table below:
Location (Room and Building)
3. Training on the use of autoclaves will consist of the following and documentation of training
will be maintained in the laboratory and will be available for review by TSO and IBC upon
request.
a. Appropriate PPE requirements such as the use of heat resistant gloves, lab coats, and
safety eye and face protection.
b. A discussion of the types of items that can and those that cannot be autoclaved.
c. Proper packaging of biohazardous wastes for autoclaving.
d. Methods for loading materials into an autoclave and unloading procedures.
e. The use of test strips and biological indicators for quality control.
f. Autoclave operational procedures including emergency shutdown precautions.
g. How to dispose of autoclaved waste.
h. Record keeping
4. Maintenance and Testing of Autoclaves
a. Department or PI responsible for autoclave must properly maintain and service. If
autoclave is owned by PI, list service provider and frequency of preventative maintenance
on autoclave:
Page 4 of 5
ISU BSL1 Laboratory Biosafety Manual/IBC
PI:
Version 1.0
b. Department or PI responsible for autoclave must occasionally quality tested to ensure
proper sterilization procedures are met and decontamination of biohazardous waste is
complete. If autoclave is owned by PI, list method of testing autoclave and frequency of
testing on autoclave:
Principal Investigator Certification
I hereby certify that I have reviewed these practices and procedures and they represent the
current operating practices in my laboratory.
Signature: Date:
Annual Review (Signature): Date:
Annual Review (Signature): Date:
Personnel Certification
We, the undersigned, have reviewed these practices and procedures, have been trained in the
appropriate methods and practices for handling potential infectious material and agree to follow
the stated practices and procedures. We understand that we must review and document
compliance with these practices and procedures on an annual basis.
Name
Signature
Date
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Completed 5 December 2019

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